The Acme Corporation is a fictional corporation that exists in several cartoons, films and TV series, most significantly in the Looney Tunes universe, where it appeared most prominently in the Road Runner/Wile E. Coyote cartoons, which made Acme famous for outlandish and downright dangerous products that failed catastrophically at the worst possible times.

The first appearance of the Acme Corporation was in Looney Tunes in a Buddy cartoon (Buddy’s Bug Hunt). It also appeared in the Egghead cartoon Count Me Out in which Egghead purchases a "Learn How To Box" kit from Acme.

The company is never clearly defined but is widely believed to be an acronym for American company making everything or A Company that Makes Everything. It appears to be a conglomerate which produces everything and anything imaginable, no matter how elaborate or extravagant. An example is the Acme Giant Rubber Band, subtitled "(For Tripping Road Runners)", which would appear to be produced specifically for Wile E. Coyote. Acme is often used whenever a cartoon, film or similar needs a corporation for a product, and instead of stealing an existing (perhaps leading to trademark issues) or making one up, they simply use Acme.